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Recombinant human trypsin

Category:Active Pharmaceutical Ingredients > Biochemical Ingredients > Enzymes
Product Name:Recombinant human trypsin
CAS No.:NA
Standard:USP, BP, EP, JP, In-house Standards
Company:Seebio Biotech (Shanghai) Co., Ltd.

Basic Info
  • Grade: Food Grade

    Factory Location: China

    Main Sales Markets: North America,Central/South America,Western Europe,Eastern Europe,Australasia,Asia,Middle East

  • Monthly Production Capacity: NA

    Packaging Information: 10mg,100mg,1g,

  • Delivery Lead Time: 10-30days

    Sample Provided: yes

    Payment Terms: TT; L/C;

    Recombinant human trypsin Reagent grade,pharmaceutical grade CAS Number 9002-07-7 EC Number 232-650-8 Package:10mg,100mg,1g, bulk Function: cell separation,degrade modified protein, treat cancer Application: various biotechnological processes Tags: Anti Tumor Promote digestion New Products.

    In the recombinant human trypsin, the sequence and the nature of amino acid is completely same with human trypsin. Trypsin is a kind of endopeptidase, it can be used to shear peptide bond of lysine and C end of arginine, thus split the macromolecular protein into small peptide.

     

    It is gene engineering produced, non animal source, no virus pollution, non hybrid enzyme pollution.

    Qualification:

    Items

    Content

    Sources

    recombinant Escherichia coli

    Purification

    HPLC

    Properties

    white or off white freeze-dried powder

    Electrophoresis and Coomassie brilliant blue staining 12%SDS-PAGE

    Non reduced SDS-PAGE protein electrophoresis, the molecular weight is24kD, a single main band.

    Specific activity

    ≥2500 USPu/mgpro. ≥3800USPu/mgpro

    Other enzyme content

    not polluted by chymotrypsin and carboxypeptidase A and activity

    It does not contain any protease inhibitors

    It doesn’t contain any PMSF, EDTA and other protease inhibitors


    Activity unit:25℃,pH7.6,reaction system 3.0ml(1cm light path),each minute emphasis BAEE make absorption value below 253nm increase 0.003 , it is a USP unit.

    Packing:10mg,100mg,1g, bulk.

    Trypsin is widely used in various biotechnological processes, such as:

    1) cell separation of various cell cultureed tissue

    2) degradation of modified protein

    3) protein enzymolysis and sequencing

    4) cells treatment of cancer, etc.

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