Tris(2-carboxyethyl)phosphine hydrochloride (TCEP hydrochloride) is a novel reducing and deprotecting agent for thiolated DNA. Thiolated DNA terminal sulfur atoms tend to form dimers in solution, especially in the presence of oxygen.

This dimerization greatly reduces the efficiency of some coupling reaction experiments (such as DNA immobilization in biosensors); while adding tris(2-carboxyethyl) phosphine hydrochloride (TCEP salt) to the DNA solution acid salt), which can be removed after a period of reaction to reduce the dimerization of DNA.

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