Hydrophobic interaction chromatography(HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.

Phenyl Bestarose HP is based on highly cross-linked agarose, which retains the excellent hydrophilicity and pore structure of natural polysaccharide compounds and has good compatibility with biological macromolecules. The average particle size of Phenyl Bestarose HP is 34μm, which has a higher resolution efficiency than Phenyl Bestarose FF. The surface contains hydrophobic ligands, the ligand is butyl, which is particularly suitable for the fine separation and purification of recombinant proteins, antibodies, vaccines, VLP particles and other highly hydrophobic biological molecules.?